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Lls had been incubated with nonenzymatic cell dissociation buffer at 37C for ten min and were scraped carefully and replated in cAMP-Screen Direct precoated 96-well assay plates at a density of 1.0 ten five cells per well to assess cAMP production. Twenty-four hours later, the medium was re- placed with FBS-free medium for 2 h, followed by 3 h of LPS stimulation. The culture medium of manage wells
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